Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments

Summary: Transfer RNA (tRNA), its post-transcriptional modifications, and tRNA-derived fragments (tRFs) play essential roles in cellular processes and gene regulation. Here, we present a fast and efficient tRNA enrichment protocol using silica spin columns. To analyze tRNA and tRFs, we describe step...

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Главные авторы:	Pavlina Gregorova, Minna-Maria K. Heinonen, Milla M. Laarne, L. Peter Sarin
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Elsevier 2026-03-01
Серии:	STAR Protocols
Предметы:	Cell Biology Molecular Biology Molecular/Chemical Probes
Online-ссылка:	http://www.sciencedirect.com/science/article/pii/S2666166726000109
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