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Metabolic Labeling of Yeast RNA with Radioactive Uracil

To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary t...

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Autor principal: Yuehua Wei
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-protocol LLC 2013-04-01
Colección:Bio-Protocol
Acceso en línea:https://bio-protocol.org/en/bpdetail?id=623&type=0
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