Metabolic Labeling of Yeast RNA with Radioactive Uracil
To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary t...
Збережено в:
| Автор: | |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Bio-protocol LLC
2013-04-01
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| Серія: | Bio-Protocol |
| Онлайн доступ: | https://bio-protocol.org/en/bpdetail?id=623&type=0 |
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