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Metabolic Labeling of Yeast RNA with Radioactive Uracil

To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary t...

Täydet tiedot

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Bibliografiset tiedot
Päätekijä: Yuehua Wei
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Bio-protocol LLC 2013-04-01
Sarja:Bio-Protocol
Linkit:https://bio-protocol.org/en/bpdetail?id=623&type=0
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