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Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp

This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of a...

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Bibliografske podrobnosti
Principais autores: Christopher Snowden, Benjamin Thomas, Charles Baxter, J. Andrew Smith, Lee Sweetlove
Format: Artigo
Jezik:Inglês
Izdano: Bio-protocol LLC 2015-12-01
Serija:Bio-Protocol
Online dostop:https://bio-protocol.org/en/bpdetail?id=1686&type=0
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