Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp
This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of a...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-protocol LLC
2015-12-01
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| coleção: | Bio-Protocol |
| Acesso em linha: | https://bio-protocol.org/en/bpdetail?id=1686&type=0 |
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