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Evaluation of Parameters in the Isolation of Viable Protoplasts from Cultured Tobacco Cells
A systematic evaluation disclosed the following conditions to be optimum for the isolation of viable protoplasts from cultured cells of Nicotiana tabacum L. `Bright Yellow' grown in liquid suspensions: (a) the cell culture in the early phase of cell number increase, (b) an enzyme mixture of 1%...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1974
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC366637/ https://ncbi.nlm.nih.gov/pubmed/16659004 |
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