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Evaluation of Parameters in the Isolation of Viable Protoplasts from Cultured Tobacco Cells

A systematic evaluation disclosed the following conditions to be optimum for the isolation of viable protoplasts from cultured cells of Nicotiana tabacum L. `Bright Yellow' grown in liquid suspensions: (a) the cell culture in the early phase of cell number increase, (b) an enzyme mixture of 1%...

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Detalhes bibliográficos
Main Authors: Uchimiya, Hirofumi, Murashige, Toshio
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC366637/
https://ncbi.nlm.nih.gov/pubmed/16659004
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