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Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp

This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of a...

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Autori principali: Christopher Snowden, Benjamin Thomas, Charles Baxter, J. Andrew Smith, Lee Sweetlove
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2015-12-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=1686&type=0
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