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PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

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Autors principals: Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
Format: Artigo
Idioma:Inglês
Publicat: MDPI AG 2022-08-01
Col·lecció:Cells
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Accés en línia:https://www.mdpi.com/2073-4409/11/16/2467
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