PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

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Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	MDPI AG 2022-08-01
Цуврал:	Cells
Нөхцлүүд:	gRNA array multiplexed CRISPR genome editing assembly method Golden Gate assembly PARA
Онлайн хандалт:	https://www.mdpi.com/2073-4409/11/16/2467
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!