PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής

Κύριοι συγγραφείς:	Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	MDPI AG 2022-08-01
Σειρά:	Cells
Θέματα:	gRNA array multiplexed CRISPR genome editing assembly method Golden Gate assembly PARA
Διαθέσιμο Online:	https://www.mdpi.com/2073-4409/11/16/2467
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