PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

Celý popis

Podrobná bibliografie

Hlavní autoři:	Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	MDPI AG 2022-08-01
Edice:	Cells
Témata:	gRNA array multiplexed CRISPR genome editing assembly method Golden Gate assembly PARA
On-line přístup:	https://www.mdpi.com/2073-4409/11/16/2467
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!