PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

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Autori principali:	Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	MDPI AG 2022-08-01
Serie:	Cells
Soggetti:	gRNA array multiplexed CRISPR genome editing assembly method Golden Gate assembly PARA
Accesso online:	https://www.mdpi.com/2073-4409/11/16/2467
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