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Replacement of Histidine 340 with Alanine Inactivates the Group A Streptococcus Extracellular Cysteine Protease Virulence Factor

Streptococcus pyogenes expresses a highly conserved extracellular cysteine protease that is a virulence factor for invasive disease, including soft tissue infection. Site-directed mutagenesis was used to generate a His340Ala recombinant mutant protein that was made as a stable 40-kDa zymogen by Esch...

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Auteurs principaux: Gubba, Siddeswar, Cipriano, Vincent, Musser, James M.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2000
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC97664/
https://ncbi.nlm.nih.gov/pubmed/10816533
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