Experimental Evolution of Enzyme Temperature Activity Profile: Selection In Vivo and Characterization of Low-Temperature-Adapted Mutants of Pyrococcus furiosus Ornithine Carbamoyltransferase

We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15°C, a temperature already in the psychrophilic r...

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Hlavní autoři: Roovers, Martine, Sanchez, Rony, Legrain, Christianne, Glansdorff, Nicolas
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2001
Témata:
Enzymes and Proteins
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC94980/
https://ncbi.nlm.nih.gov/pubmed/11208811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.3.1101-1105.2001
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