Experimental Evolution of Enzyme Temperature Activity Profile: Selection In Vivo and Characterization of Low-Temperature-Adapted Mutants of Pyrococcus furiosus Ornithine Carbamoyltransferase

We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15°C, a temperature already in the psychrophilic r...

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Detalhes bibliográficos
Main Authors: Roovers, Martine, Sanchez, Rony, Legrain, Christianne, Glansdorff, Nicolas
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC94980/
https://ncbi.nlm.nih.gov/pubmed/11208811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.3.1101-1105.2001
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