Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was i...

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Pubblicato in:	J Bacteriol
Autori principali:	Monchois, Vincent, Arguello-Morales, Martha, Russell, Roy R. B.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93647/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10094712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.7.2290-2292.1999
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