Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase
Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was i...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93647/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10094712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.7.2290-2292.1999 |
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