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Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was i...

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Detalhes bibliográficos
Main Authors: Monchois, Vincent, Arguello-Morales, Martha, Russell, Roy R. B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93647/
https://ncbi.nlm.nih.gov/pubmed/10094712
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