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Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was i...

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Udgivet i:J Bacteriol
Principais autores: Monchois, Vincent, Arguello-Morales, Martha, Russell, Roy R. B.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Enzymes and Proteins
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93647/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10094712/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.7.2290-2292.1999
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