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An rpsL Cassette, Janus, for Gene Replacement through Negative Selection in Streptococcus pneumoniae

Natural genetic transformation offers a direct route by which synthetic gene constructs can be placed into the single circular chromosome of Streptococcus pneumoniae. However, the lack of a general negative-selection marker has hampered the introduction of constructs that do not confer a selectable...

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Bibliografische gegevens
Hoofdauteurs: Sung, C. K., Li, H., Claverys, J. P., Morrison, D. A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2001
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC93289/
https://ncbi.nlm.nih.gov/pubmed/11679344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.11.5190-5196.2001
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