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Use of a Dominant rpsL Allele Conferring Streptomycin Dependence for Positive and Negative Selection in Thermus thermophilus

A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the a...

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Autori principali: Blas-Galindo, Emilio, Cava, Felipe, López-Viñas, Eduardo, Mendieta, Jesús, Berenguer, José
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950985/
https://ncbi.nlm.nih.gov/pubmed/17601820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00751-07
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