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An rpsL Cassette, Janus, for Gene Replacement through Negative Selection in Streptococcus pneumoniae
Natural genetic transformation offers a direct route by which synthetic gene constructs can be placed into the single circular chromosome of Streptococcus pneumoniae. However, the lack of a general negative-selection marker has hampered the introduction of constructs that do not confer a selectable...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC93289/ https://ncbi.nlm.nih.gov/pubmed/11679344 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.11.5190-5196.2001 |
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