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A Modified Janus Cassette (Sweet Janus) to Improve Allelic Replacement Efficiency by High-Stringency Negative Selection in Streptococcus pneumoniae

The Janus cassette permits marker-free allelic replacement or knockout in streptomycin-resistant Streptococcus pneumoniae (pneumococcus) through sequential positive and negative selection. Spontaneous revertants of Janus can lead to high level of false-positives during negative selection, which nece...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Li, Yuan, Thompson, Claudette M., Lipsitch, Marc
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Public Library of Science 2014
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4068995/
https://ncbi.nlm.nih.gov/pubmed/24959661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0100510
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