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L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs)

A flexible, non-gel-based single nucleotide polymorphism (SNP) detection method is described. The method adopts thermostable ligation for allele discrimination and rolling circle amplification (RCA) for signal enhancement. Clear allelic discrimination was achieved after staining of the final reactio...

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Main Authors: Qi, Xiaoquan, Bakht, Saleha, Devos, Katrien M., Gale, Mike D., Osbourn, Anne
格式: Artigo
語言:Inglês
出版: Oxford University Press 2001
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC92587/
https://ncbi.nlm.nih.gov/pubmed/11713336
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