L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs)

A flexible, non-gel-based single nucleotide polymorphism (SNP) detection method is described. The method adopts thermostable ligation for allele discrimination and rolling circle amplification (RCA) for signal enhancement. Clear allelic discrimination was achieved after staining of the final reactio...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Qi, Xiaoquan, Bakht, Saleha, Devos, Katrien M., Gale, Mike D., Osbourn, Anne
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92587/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11713336/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.22.e116
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