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L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs)

A flexible, non-gel-based single nucleotide polymorphism (SNP) detection method is described. The method adopts thermostable ligation for allele discrimination and rolling circle amplification (RCA) for signal enhancement. Clear allelic discrimination was achieved after staining of the final reactio...

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Hlavní autoři: Qi, Xiaoquan, Bakht, Saleha, Devos, Katrien M., Gale, Mike D., Osbourn, Anne
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC92587/
https://ncbi.nlm.nih.gov/pubmed/11713336
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