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Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein

Rolling circle amplification (RCA) of plasmid or genomic DNA using random hexamers and bacteriophage phi29 DNA polymerase has become increasingly popular in the amplification of template DNA in DNA sequencing. We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Th...

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Detalhes bibliográficos
Main Authors: Inoue, Jin, Shigemori, Yasushi, Mikawa, Tsutomu
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1463899/
https://ncbi.nlm.nih.gov/pubmed/16707659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl350
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