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Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein

Rolling circle amplification (RCA) of plasmid or genomic DNA using random hexamers and bacteriophage phi29 DNA polymerase has become increasingly popular in the amplification of template DNA in DNA sequencing. We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Th...

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Autores principales: Inoue, Jin, Shigemori, Yasushi, Mikawa, Tsutomu
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2006
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1463899/
https://ncbi.nlm.nih.gov/pubmed/16707659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl350
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