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Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products
In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with grea...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1183492/ https://ncbi.nlm.nih.gov/pubmed/16087733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni111 |
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