Selective and Sensitive Method for PCR Amplification of Escherichia coli 16S rRNA Genes in Soil

A set of PCR primers targeting 16S rRNA gene sequences was designed, and PCR parameters were optimized to develop a robust and reliable protocol for selective amplification of Escherichia coli 16S rRNA genes. The method was capable of discriminating E. coli from other enteric bacteria, including its...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	Appl Environ Microbiol
Päätekijät:	Sabat, G., Rose, P., Hickey, W. J., Harkin, J. M.
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 2000
Aiheet:	Methods
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10653763/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.2.844-849.2000
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