Selective and Sensitive Method for PCR Amplification of Escherichia coli 16S rRNA Genes in Soil

A set of PCR primers targeting 16S rRNA gene sequences was designed, and PCR parameters were optimized to develop a robust and reliable protocol for selective amplification of Escherichia coli 16S rRNA genes. The method was capable of discriminating E. coli from other enteric bacteria, including its...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Sabat, G., Rose, P., Hickey, W. J., Harkin, J. M.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2000
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10653763/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.2.844-849.2000
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