Selective and Sensitive Method for PCR Amplification of Escherichia coli 16S rRNA Genes in Soil
A set of PCR primers targeting 16S rRNA gene sequences was designed, and PCR parameters were optimized to develop a robust and reliable protocol for selective amplification of Escherichia coli 16S rRNA genes. The method was capable of discriminating E. coli from other enteric bacteria, including its...
Guardado en:
| Publicado en: | Appl Environ Microbiol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2000
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10653763/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.2.844-849.2000 |
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