Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR

A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with the use of this gene as an amplification ta...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Kaczmarski, E. B., Fox, A. J.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2000
נושאים:
Bacteriology
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC86577/
https://ncbi.nlm.nih.gov/pubmed/10790092
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