Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR

A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with the use of this gene as an amplification ta...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Kaczmarski, E. B., Fox, A. J.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2000
Schlagworte:	Bacteriology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.1747-1752.2000
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