Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with the use of this gene as an amplification ta...
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| Pubblicato in: | J Clin Microbiol |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
2000
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.1747-1752.2000 |
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