Peptide Nucleic Acid-Mediated PCR Clamping as a Useful Supplement in the Determination of Microbial Diversity

Peptide nucleic acid (PNA)-mediated PCR clamping (H. Ørum, P. E. Nielsen, M. Egholm, R. H. Berg, O. Buchardt, and C. Stanley, Nucleic Acids Res. 21:5332–5336, 1993) was introduced as a novel procedure to selectively amplify ribosomal DNAs (rDNAs) which are not frequently found in clone libraries gen...

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Main Authors: von Wintzingerode, Friedrich, Landt, Olfert, Ehrlich, Angelika, Göbel, Ulf B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC91862/
https://ncbi.nlm.nih.gov/pubmed/10653717
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