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Peptide Nucleic Acid-Mediated Competitive PCR Clamping for Detection of Rifampin-Resistant Mycobacterium tuberculosis

Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 o...

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Detalhes bibliográficos
Main Authors: Iwamoto, Tomotada, Sonobe, Toshiaki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC521877/
https://ncbi.nlm.nih.gov/pubmed/15388471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.10.4023-4026.2004
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