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Peptide Nucleic Acid-Mediated Competitive PCR Clamping for Detection of Rifampin-Resistant Mycobacterium tuberculosis

Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 o...

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書目詳細資料
Main Authors: Iwamoto, Tomotada, Sonobe, Toshiaki
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC521877/
https://ncbi.nlm.nih.gov/pubmed/15388471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.10.4023-4026.2004
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