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Detection of Viable Listeria monocytogenes with a 5′ Nuclease PCR Assay

A 5′ nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose act...

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Bibliografiske detaljer
Main Authors: Norton, Dawn-Marie, Batt, Carl A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91307/
https://ncbi.nlm.nih.gov/pubmed/10224010
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