Detection of Viable Listeria monocytogenes with a 5′ Nuclease PCR Assay
A 5′ nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose act...
Gespeichert in:
| Veröffentlicht in: | Appl Environ Microbiol |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1999
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91307/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10224010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.2122-2127.1999 |
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