Quantification of Hepatitis A Virus in Shellfish by Competitive Reverse Transcription-PCR with Coextraction of Standard RNA

To quantify hepatitis A virus (HAV) in experimentally contaminated mussels, we developed an internal standard RNA with a 7-nucleotide deletion for competitive reverse transcription (RT)-PCR. Deposited directly into the sample, this standard was used both as extraction control and as quantification t...

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में प्रकाशित:	Appl Environ Microbiol
मुख्य लेखकों:	Arnal, Charlotte, Ferre-Aubineau, Virginie, Mignotte, Berangere, Imbert-Marcille, Berthe Marie, Billaudel, Sylviane
स्वरूप:	Artigo
भाषा:	Inglês
प्रकाशित:	American Society for Microbiology (ASM) 1999
विषय:	Environmental and Public Health Microbiology
ऑनलाइन पहुंच:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91024/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9872801/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.1.322-326.1999
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