Reverse Transcriptase PCR Detection of Astrovirus, Hepatitis A Virus, and Poliovirus in Experimentally Contaminated Mussels: Comparison of Several Extraction and Concentration Methods

Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with astrovirus, hepatitis A virus, or poliovirus. Sixty-...

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Main Authors: Traore, Ousmane, Arnal, Charlotte, Mignotte, Berengere, Maul, Armand, Laveran, Henri, Billaudel, Sylviane, Schwartzbrod, Louis
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1998
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Methods
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC106830/
https://ncbi.nlm.nih.gov/pubmed/9687488
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