Reverse Transcriptase PCR Detection of Astrovirus, Hepatitis A Virus, and Poliovirus in Experimentally Contaminated Mussels: Comparison of Several Extraction and Concentration Methods

Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with astrovirus, hepatitis A virus, or poliovirus. Sixty-...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Traore, Ousmane, Arnal, Charlotte, Mignotte, Berengere, Maul, Armand, Laveran, Henri, Billaudel, Sylviane, Schwartzbrod, Louis
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106830/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9687488/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.8.3118-3122.1998
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