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Quantification of Hepatitis A Virus in Shellfish by Competitive Reverse Transcription-PCR with Coextraction of Standard RNA

To quantify hepatitis A virus (HAV) in experimentally contaminated mussels, we developed an internal standard RNA with a 7-nucleotide deletion for competitive reverse transcription (RT)-PCR. Deposited directly into the sample, this standard was used both as extraction control and as quantification t...

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Detalles Bibliográficos
Main Authors: Arnal, Charlotte, Ferre-Aubineau, Virginie, Mignotte, Berangere, Imbert-Marcille, Berthe Marie, Billaudel, Sylviane
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC91024/
https://ncbi.nlm.nih.gov/pubmed/9872801
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