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Isolation of Rat Dihydrofolate Reductase Gene and Characterization of Recombinant Enzyme
While assays of many antifolate inhibitors for dihydrofolate reductase (DHFR) have been performed using rat DHFR as a target, neither the sequence nor the structure of rat DHFR is known. Here, we report the isolation of the rat DHFR gene through screening of a rat liver cDNA library. The rat liver D...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2001
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC90686/ https://ncbi.nlm.nih.gov/pubmed/11502523 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.9.2517-2523.2001 |
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