Isolation of Rat Dihydrofolate Reductase Gene and Characterization of Recombinant Enzyme

While assays of many antifolate inhibitors for dihydrofolate reductase (DHFR) have been performed using rat DHFR as a target, neither the sequence nor the structure of rat DHFR is known. Here, we report the isolation of the rat DHFR gene through screening of a rat liver cDNA library. The rat liver D...

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Detalhes bibliográficos
Main Authors: Wang, Yangzhou, Bruenn, Jeremy A., Queener, Sherry F., Cody, Vivian
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Mechanisms of Action: Physiological Effects
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC90686/
https://ncbi.nlm.nih.gov/pubmed/11502523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.9.2517-2523.2001
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