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Purification, Reconstitution, and Inhibition of Cytochrome P-450 Sterol Δ(22)-Desaturase from the Pathogenic Fungus Candida glabrata

Sterol Δ(22)-desaturase has been purified from a strain of Candida glabrata with a disruption in the gene encoding sterol 14α-demethylase (cytochrome P-45051; CYP51). The purified cytochrome P-450 exhibited sterol Δ(22)-desaturase activity in a reconstituted system with NADPH–cytochrome P-450 reduct...

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Détails bibliographiques
Auteurs principaux: Lamb, David C., Maspahy, Segula, Kelly, Diane E., Manning, Nigel J., Geber, Antonia, Bennett, John E., Kelly, Steven L.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 1999
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC89351/
https://ncbi.nlm.nih.gov/pubmed/10390230
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