DNA Cleavage Activities of Staphylococcus aureus Gyrase and Topoisomerase IV Stimulated by Quinolones and 2-Pyridones

We have cloned Staphylococcus aureus DNA gyrase and topoisomerase IV and expressed them in Escherichia coli as polyhistidine-tagged proteins to facilitate purification and eliminate contamination by host enzymes. The enzyme preparations had specific activities similar to previously reported values....

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Main Authors: Saiki, Anne Y. C., Shen, Linus L., Chen, Chih-Ming, Baranowski, John, Lerner, Claude G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
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Mechanisms of Action: Physiological Effects
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC89326/
https://ncbi.nlm.nih.gov/pubmed/10390205
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