DNA Cleavage Activities of Staphylococcus aureus Gyrase and Topoisomerase IV Stimulated by Quinolones and 2-Pyridones

We have cloned Staphylococcus aureus DNA gyrase and topoisomerase IV and expressed them in Escherichia coli as polyhistidine-tagged proteins to facilitate purification and eliminate contamination by host enzymes. The enzyme preparations had specific activities similar to previously reported values....

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Εκδόθηκε σε:	Antimicrob Agents Chemother
Κύριοι συγγραφείς:	Saiki, Anne Y. C., Shen, Linus L., Chen, Chih-Ming, Baranowski, John, Lerner, Claude G.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 1999
Θέματα:	Mechanisms of Action: Physiological Effects
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC89326/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10390205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.43.7.1574
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