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Streptococcus pneumoniae DNA Gyrase and Topoisomerase IV: Overexpression, Purification, and Differential Inhibition by Fluoroquinolones

Streptococcus pneumoniae gyrA and gyrB genes specifying the DNA gyrase subunits have been cloned into pET plasmid vectors under the control of an inducible T7 promoter and have been separately expressed in Escherichia coli. Soluble 97-kDa GyrA and 72-kDa GyrB proteins bearing polyhistidine tags at t...

詳細記述

保存先:
書誌詳細
主要な著者: Pan, Xiao-Su, Fisher, L. Mark
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 1999
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC89122/
https://ncbi.nlm.nih.gov/pubmed/10223925
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