Streptococcus pneumoniae DNA Gyrase and Topoisomerase IV: Overexpression, Purification, and Differential Inhibition by Fluoroquinolones

Streptococcus pneumoniae gyrA and gyrB genes specifying the DNA gyrase subunits have been cloned into pET plasmid vectors under the control of an inducible T7 promoter and have been separately expressed in Escherichia coli. Soluble 97-kDa GyrA and 72-kDa GyrB proteins bearing polyhistidine tags at t...

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Pubblicato in:	Antimicrob Agents Chemother
Autori principali:	Pan, Xiao-Su, Fisher, L. Mark
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Mechanisms of Resistance
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC89122/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10223925/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.43.5.1129
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