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Streptococcus pneumoniae DNA Gyrase and Topoisomerase IV: Overexpression, Purification, and Differential Inhibition by Fluoroquinolones

Streptococcus pneumoniae gyrA and gyrB genes specifying the DNA gyrase subunits have been cloned into pET plasmid vectors under the control of an inducible T7 promoter and have been separately expressed in Escherichia coli. Soluble 97-kDa GyrA and 72-kDa GyrB proteins bearing polyhistidine tags at t...

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Bibliografski detalji
Glavni autori: Pan, Xiao-Su, Fisher, L. Mark
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC89122/
https://ncbi.nlm.nih.gov/pubmed/10223925
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